Electrophoresis

Electrophoresis is an analytical method in which a controlled electric current is used in order to separate biomolecules according to their size to electric charge ratio, using a gelatinous matrix as a base.

Electrophoresis is used in a great majority in the field of recombinant DNA since it allows us to know the charge that polypeptides have, and to separate the different polypeptides resulting from the variations of the recombinant DNA experiment.

The most commonly used variant for the analysis of mixtures of proteins or nucleic acids uses a gel as a support, usually made of agarose or polyacrylamide. Nucleic acids already have a negative electrical charge, which will direct them to the positive pole, while proteins become charged by binding with substances such as SDS that incorporate negative charges in a way that is dependent on the molecular mass of the protein.

By putting the mixture of molecules and applying an electric field, they will move and must go through the gel mesh (a three-dimensional network of crossed fibers), so that the small ones will move better, faster. Thus, the smallest will advance further and the largest will be close to the starting point.

Horizontal and vertical electrophoresis: Differences

Horizontal gel electrophoresis uses the basic theory for the separation of DNA, RNA or protein molecules according to their respective molecular size and charge. In this technique, the gel is present in a horizontal orientation and is immersed in a buffer that is continuous. The agarose gel is used to separate the gel box into two compartments. One end of the gel box contains an anode, while the other end contains a cathode. When a current is applied, the buffer used in this technique allows the creation of a charge gradient. When the load is applied, the gel tends to heat up.

Electrophoresis and Gel Documentation Systems

Gel electrophoresis is a widely used technique in life science laboratories to separate macromolecules such as DNA, RNA, and proteins. In this technique, molecules are separated according to size and electrical charge.

How does electrophoresis work?

The gel used in gel electrophoresis is generally made from a material called agarose, which is a gelatinous substance extracted from seaweed. This porous gel could be used to separate macromolecules of many different sizes.

Types of Electrophoresis

At Kalstein we have an excellent range of laboratory electrophoresis. That is why we invite you to see our product section in the menu.

 
 
Horizontal Gel Electrophoresis

Horizontal gel electrophoresis uses the basic theory for the separation of DNA, RNA or protein molecules according to their respective molecular size and charge. In this technique, the gel is present in a horizontal orientation and is immersed in a buffer that is continuous.

Vertical Gel Electrophoresis

The vertical gel electrophoresis technique works according to the primary theory of gel electrophoresis, but is considered to be more complex than the horizontal gel electrophoresis method.

KALSTEIN ELECTROPHORESIS SYSTEMS

At Kalstein you can find the ideal electrophoresis systems for your laboratory.

Electrophoresis is a common technique in the clinical laboratory. It allows the separation of chemical species (nucleic acids or proteins) along an electric field based on their size and their electric charge.

Capillary electrophoresis

Capillary electrophoresis is a separation technique used in different areas to separate the different molecules present in a solution, allowing results to be obtained in a short time

Agarose gel electrophoresis

Agarose gel electrophoresis is the standard method to separate and purify DNA fragments when high resolving power is not required.

Polyacrylamide Gel Electrophoresis

Polyacrylamide gel electrophoresis is one of the most widely used methods for the purification, analysis and characterization of proteins in laboratories.

Our Best Selling Electrophoresis System

  • Installing the glass plate in just 15 seconds, and you don't need the button, it's quick and convenient.
  • With a gel mold in its original position, it is not necessary to move the vessels from gel fabrication to electrophoresis. It is more convenient to observe the gel preparation on both sides of the vessels.
  • High transparency polycarbonate material injection molding, impact resistance, high temperature resistance, corrosion resistance.
  • Safety cover open button design, convenient cover opening.

KALSTEIN UPDATED

What is your ideal laboratory Electrophoresis System?

There are countless models, so it is normal that you do not know which laboratory electrophoresis to buy that suits your needs. At Kalstein, we test them to find what you're looking for.

 

Electrophoresis: what are the necessary reagents?

Electrophoresis is an analytical technique in which a controlled electric current is used in order to separate biomolecules according to their size-to-electric charge ratio.

Gel documentation systems

Gel electrophoresis is a widely used technique in life science laboratories to separate macromolecules

What is electrophoresis and why is it important?

Electrophoresis is an analytical method in which a controlled electric current is used in order to separate biomolecules according to their size-to-electric charge ratio.

Hemoglobin electrophoresis and hemoglobinopathies

In a generic way, the clinical syndromes that result from alterations in hemoglobin (Hb) are known as hemoglobinopathies and various laboratory tests are interpreted to determine them, of which the most relevant is Hb electrophoresis (EF).

Electrophoresis Systems on Offer

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If you need a quote, have a question about any product and / or want to clarify any doubts, please contact us.

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