Vertical Gel Electrophoresis

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April 18, 2022

by Kalstein

<strong>Manufacturer</strong>: <em>Kalstein</em> <img class="alignnone size-full wp-image-7597" src="https://www.kalstein.site/wp-content/uploads/2020/01/CE.jpg" alt="" width="54" height="53" />

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December 15, 2022

by Kalstein

Electrophoresis is a basic method in the field of molecular biology for the analysis (separation, purification, preparation) of nucleic acids and proteins. The principle of electrophoresis consists in the migration of the molecules through a gel or another type of matrix of a porous nature, in which, by the action of an electric field, they will be separated according to their size or molecular weight, this is achieved thanks to the action of a power source for electrophoresis.

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December 15, 2022

by Kalstein

A power source for electrophoresis is the source that manages constant and direct current to the electrophoresis system, as well as indicates and allows us to control both the supply voltage and the current consumption. In other words, this device provides the necessary energy for this system to function properly; and this important laboratory technique can be carried out.

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December 15, 2022

by Kalstein

The power source for electrophoresis chambers is the source in charge of providing electricity to the electrophoresis system that is being used, in other words, it provides the necessary energy for this system to function properly; reason for which it is considered an essential element to carry out the electrophoresis technique.

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December 12, 2022

by Kalstein

Gel electrophoresis is a laboratory technique used in genetics to separate mixtures containing DNA, RNA, and other proteins according to their respective molecular size and charge. Gel electrophoresis can be of two different methods: horizontal gel electrophoresis and vertical gel electrophoresis.

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December 12, 2022

by Kalstein

The electrophoresis chamber is the device where the sample is introduced for said process; and where the electromagnetic field that is formed in the electrophoresis process is created, said field takes place within a buffer solution in which the gel is submerged; the high concentration of electrolytes makes the transition of electric current possible. The principle of electrophoresis consists in the migration of the molecules through the gel generated by the electromagnetic field according to the molecular weight and size. This gel has pores that act like a strainer, causing small molecules to move faster than large molecules. In the chamber there are two poles that connect to the power source.

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December 12, 2022

by Kalstein

This technique is widely used in laboratories, especially in those of molecular biology, because it is used in important procedures such as: separation, analysis and purification of RNA, DNA, or proteins, nucleic acids, this process is performed because most biomolecules have an electrical charge where their magnitude depends on the pH of the medium in which they are found; because of this, the biomolecules move when subjected to an electric field to the charge pole opposite to that of the molecule.

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